Serum vitamin D levels in acute stroke patients

Abstract

Background

Vitamin D deficiency has been proposed as a risk factors of cerebrovascular stroke.

Objectives

The aim of this study was firstly, to assess the serum level of vitamin D in cerebral stroke patients and secondly, to examine if its deficiency was associated with stroke severity and outcome.

Methods

We utilized a case-control study design and recruited 138 acute stroke patients and 138 age- and sex-matched controls from subjects attending outpatient clinic for other reasons. All participants were subjected to full general and neurological examination. Brain imaging CT and/or MRI was performed. Blood samples were collected for measurement of serum level of vitamin D (ng/ml) by ELISA, alkaline phosphatase, serum calcium, and phosphorous. The stroke severity was assessed by the National Institutes of Health Stroke Scale (NIHSS) and stroke outcome was assessed by modified Rankin Scale (mRS).

Results

Stroke patients had significant lower levels of vitamin D compared with the control group. Vitamin D deficiency remained significantly associated with the NIHSS stroke severity score and the mRS 3-month stroke outcome after controlling for other significant factors such as age, dyslipidemia, and infarction size using multivariable logistic regression analysis.

Conclusion

Our results demonstrated that stroke patients suffer from vitamin D deficiency, which was associated with both stroke severity and poor outcome. Vitamin D supplementation could exert a therapeutic role in the management of cerebral stroke.

Introduction

Vitamin D is one of the fat-soluble steroid hormones which is responsible for calcium and phosphate homeostasis and musculoskeletal health. Vitamin D is synthesized from 7-dehydrocholesterol in the skin’s epidermal layer in the presence of ultraviolet light. Then, undergo two hydroxylation steps: first inactive vitamin D is hydroxylated in the liver to form 25-hydroxyvitamin D, then classically in the kidney by 1-α-hydroxylase (CYP27B) to become biologically active form 1,25-dihydroxyvitamin D₃. The second hydroxylation step can also occur in macrophages, T cells, and neurons [1, 2]. Vitamin D has an important regulatory effect on immune function and inflammation [3]. Low vitamin D levels were associated with many illnesses such as calcium metabolism disorders, type 2 diabetes mellitus, autoimmune diseases, cardiovascular disease, stroke, multiple sclerosis, and some infectious diseases and cancers [4, 5].

Stroke is one of the most frequent causes of death and disability worldwide with a significant clinical and socioeconomic impact. It has a heterogeneous etiology including unmodifiable risk factors such as genetic, age and sex, and modifiable risk factors including hypertension, diabetes mellitus, dyslipidemia, sedentary lifestyle, and smoking [6]. Low serum vitamin D levels have been found in acute stroke patients compared with normal controls [6, 7]. Vitamin D is essential for regulation of brain growth, function, and cerebrovascular physiology [8]. It has been linked to vasoprotective potential including slowing down of atherosclerosis, promotion of endothelial function, suppression of renin–angiotensin–aldosterone system thereby reduction of the risk of hypertension. Therefore, its deficiency might be involved in the development of several diseases, including diabetes, hypertension, heart failure, ischemic heart diseases, and stroke [9]. Moreover, deficiency of vitamin D influences vascular remodeling through modulation of smooth muscle cell proliferation, inflammation, and thrombosis. These vascular changes can eventually cause stroke [10].

However, results of different researches showed that vitamin D deficiency is a risk factor of stroke; the association between the severity and outcome of stroke with the level of vitamin D has not been examined closely. The aim of this study is to evaluate the serum vitamin D levels in cerebral stroke patients and assesses association between vitamin D deficiency with severity and outcome of stroke.

Subjects and methods

Sample size

The sample size of the current case-control study was calculated based on the previously reported vitamin D deficiency rate of 48.8% in stroke patients and 31.6% in control subjects [6]. At 5% two-sided level of significance, we found that a sample size of 260 subjects (130 subjects/group) to achieve a power of 80% using the Kelsey method, Epi Info, version 7.2 (CDC, 2018). We recruited 138 subjects for each of the case and control groups.

Study design and subject recruitment

We utilized a case-control study design that included 138 cerebral stroke patients and 138 control subjects from patients attending outpatient clinics for other reasons. Patients and control subjects were matched for sex and age ± 5 years. Systemic random technique, recruiting every 3rd subject, was used for randomly selecting patients and control. Stroke patients were recruited from intensive care and stroke units of Internal Medicine and Neurology Departments, Zagazig University, between June 2016 and June 2017. All participants were informed with the study design and a written informed consent was collected from all participants or their relatives. Approval for performing the study was obtained from Institutional Review Board (IRB) of Zagazig University.

Inclusion and exclusion criteria

Any stroke patients with first-ever acute onset stroke were eligible for the study. We excluded patients with the following criteria: (1) patients with history of hepatic and renal impairment; (2) patients with brain neoplasm or malignancy; (3) patients with endocrinal diseases; (4) patients with vitamin D or Ca supplementation and steroid therapy; and (5) patients with bone diseases. Control included 138 healthy volunteers that were age and sex matched with no prior history of stroke or transient ischemic attacks.

Brain imaging

All patients were submitted to computed tomography (CT) using GE ProSpeed Dual Slice F II CT with MX135 Tube (Cleveland, USA) and/or magnetic resonance imaging (MRI) using 1.5 T MR Scanner (Achieva, Philips Medical System) of the brain on admission to differentiate patients with cerebral infarction from hemorrhage and to determine the site and size of the lesion. When there was no positive data on the admission CT scan, another CT scan or MRI was performed. The infarct size was determined by the largest diameter of the lesion [11].

Laboratory investigations

One venous blood sample was obtained for each subject. Blood samples were obtained within 48 h of the onset of stroke. Complete blood count (CBC), random blood glucose level, liver chemistry tests, kidney function tests, C-reactive protein (CRP), arterial blood gases, serum sodium and potassium, erythrocyte sedimentation rate (ESR), lipid profile (total cholesterol, triglycerides, HDL, and LDL), alkaline phosphatase, serum calcium and phosphorous, and vitamin D were performed for all participant. Measurement of serum level of vitamin D (ng/ml) was done using enzyme-linked immunosorbent assay (ELISA) following manufacturer’s instruction of the Kit (Immunodiagnostic Systems Ltd, Bolden, UK). The assay utilize as monoclonal antibody that binds to 25-OH vitaminD₃. Vitamin D deficiency was defined if its level was < 20 ng/ml [6].

Stroke severity and outcome assessment

The severity of the stroke was assessed by the National Institutes of Health Stroke Scale (NIHSS) [12]. Stroke severity was categorized as mild (NIHSS score < 8), moderate (NIHSS score 8–14), and severe stroke (NIHSS score ≥ 15) [13]. Short-term functional outcome was measured using modified Rankin Scale (mRS) after 3-months. mRS score of ≤ 2 was considered as good functional outcome while poor functional outcome was defined as mRS score of ≥ 3 [14].

Other measures

All patients of this study were subjected to detailed history taking with paying special attention to medical history of stroke risk factors, according to criteria determined by Mouradian and colleagues [15]. Full general and neurological examinations including Glasgow Coma Scale (GCS) were performed. Obesity is defined as body mass index ≥ 30 kg/m² [16]. Chest X-ray, electrocardiography, and echocardiography were done for patients with cardiac lesions.

Statistical analysis

Data were analyzed using the Statistical Package for the Social Sciences (SPSS) version 20 (IBM corporation, Armonk, New York) [17]. Quantitative variables were expressed as mean ± standard deviation (SD), whereas qualitative variables were expressed as frequencies and percentages. Comparison between patients and controls was performed using Student t test or chi-square test as deemed appropriate. Univariate followed by multivariable regression analysis was performed to identify independent significant factors associated with stroke severity and outcome. Measures of association were expressed as odds ratios (ORs) and 95% confidence interval (CI). p values ≤ 0.05 and ≤ 0.01 were considered significant and highly significant, respectively.

Partial correlation, after controlling of other significant risk factors, was performed to examine the association between vitamin D and NIHSS, and mRS scores was performed. We assessed the vitamin D cutoff levels associated with bad stroke severity and stroke outcome using the receiver-operating characteristic (ROC) analysis. Best sensitivity and specificity associated with cutoff levels was presented. ROC curve analysis was performed using the MedCalc software version 7.50 (Mariakerke, Belgium).

Results

This case-control study included 138 patients with a mean age (± SD) of 64.27 years (± 12.36), and 138 control subject with a mean age (± SD) of 64.65 years (± 10.2). According to stroke type, 30 (21.7%) of our stroke patients had intracerebral hemorrhage, and 108 patients (78.3%) had ischemic stroke (27.6% had lacunar infarction, 32% had medium-sized infarction, whereas only 18.7% presented with large infarction).

There were no statistically significant differences regarding age and sex. As regard to other risk factors, diabetes mellitus (DM) was significantly more common in stroke patients (59.4%) followed by hypertension (58.7%), obesity (42%), dyslipidemia (40.6%), smoking (33.3%), atrial fibrillation (AF) (32.6%), peripheral vascular disease (21.7%), and ischemic heart disease (20.3%) when compared with controls (31.9%, 14.5%, 28.9%, 13%, 8.7%, 7.2%, 3.6%, and 8.7% respectively). Our studied stroke patients had statistically significant lower levels of vitamin D when compared with the control group. However, serum total Ca, phosphorus, and alkaline phosphatase showed no statistically significant difference between patients and control (Table 1).

Table 1 Baseline characteristics of patients and control

Stroke severity was assessed using NIHSS. Univariable analysis showed that age, hypertension, diabetes mellitus, dyslipidemia, AF, vitamin D deficiency, CRP, large infarction size, and intracerebral hemorrhage were significantly associated with stroke severity. Further multivariable analysis of the significant variables showed that old age (OR = 1.072), dyslipidemia (OR = 3.588), vitamin D deficiency (OR = 4.790), and large infarction size (OR = 7.462) was independently associated with stroke severity. The odds to have severe stroke is 1 with old age (OR = 1.072), 3 with dyslipidemia (OR = 3.588), and increased to be almost 5 with vitamin D deficiency (OR = 4.790), and 7 with large infarction size (OR = 7.462) (Table 2).

Table 2 Univariable and Multivariable Logistic Regression analysis of factors with stroke severity (NIHSS)

As regards stroke outcome using mRS, hypertension, diabetes mellitus, dyslipidemia, vitamin D deficiency, CRP, GCS, NIHSS, large infarction size, and intracerebral hemorrhage were significantly associated with bad outcome. Multivariable analysis was used to analyze the association of significant factors with stroke outcome in univariable analysis. NIHSS score, vitamin D deficiency, and large infarction size were significant independent risk factors associated with stroke outcome. The odds to have bad outcome increase from 1 with high NIHSS score (OR = 1.094) to 2.8 with vitamin D deficiency (OR = 2.899) and 3.7 with large infarction size (OR = 3.774) (Table 3).

Table 3 Univariable and multivariable logistic regression analysis of factors with stroke outcome (mRS)

Partial correlation of vitamin D serum level with NIHSS stroke severity score after controlling for age, dyslipidemia, and infarction size was done, and a significant negative correlation was found between vitamin D serum level and NIHSS (p = 0.002, r = − 0.267) (Fig. 1). Also, partial correlation of vitamin D serum level with mRS stroke outcome score after controlling for NIHSS and infarction size was done and a significant negative correlation was found between serum vitamin D level and mRS (p = 0.01, r = − 0.207) (Fig. 2).

Fig. 1

Partial correlation analysis after controlling for age, dyslipidemia, and infarction size shows that vitamin D serum level (ng/ml) demonstrated significant negative correlation with NIHSS stroke severity score

Fig. 2

Partial correlation analysis after controlling for NIHSS score and infarction size shows that vitamin D serum level (ng/ml) demonstrated significant negative correlation with mRS stroke outcome score

Receiver operator characteristics (ROC) curve analysis of vitamin D serum level that best predict stroke severity as determined by NIHSS score was used. Vitamin D serum level of ≤ 9 ng/ml was shown to predict stroke severity with sensitivity of 74.2% and specificity of 71%. The overall performance of vitamin D marker was 77% as determined by area under curve (AUC) and was significantly different from the 50% chance diagonal line (p < 0.0001) (Fig. 3).

Fig. 3

Receiver operator characteristics (ROC) analysis showed that vitamin D serum level ≤ 9 ng/ml best predict stroke severity (as determined by NIHSS score) with sensitivity and specificity of 74.2% and 71%, respectively. Area under curve (AUC) of 77% was significantly different from the random 50% (p < 0.0001)

Moreover, using the ROC curve analysis, we tested the vitamin D serum level that best predict stroke outcome as determined by mRS score. Vitamin D serum level of ≤ 17 ng/ml was shown to predict stroke outcome with sensitivity of 81.4% but with low specificity of 42.7%. The overall performance of vitamin D marker was 65.4% as determined by area under curve (AUC) that was significantly different from the 50% chance diagonal line (p < 0.001) (Fig. 4).

Fig. 4

Receiver operator characteristics (ROC) analysis showed that vitamin D serum level − 17 ng/ml that best predict stroke outcome (as determined by mRS score) with sensitivity and specificity of 81.4% and 42.7%, respectively. Area under curve (AUC) was 65.4% was significantly different from the random 50% (p < 0.001)

Discussion

In the current study, the most prevalent risk factors of stroke were all modifiable. Diabetes mellitus was the most common followed by hypertension, obesity, dyslipidemia, smoking, AF, peripheral vascular disease, ischemic heart disease, and vitamin D deficiency. In general, this result was in agreement with the results derived from many previous epidemiological studies [18, 19]. Strong evidence for the association of low vitamin D with higher risk of cerebral stroke stems from several meta-analyses studies [20, 21]. The incidence of cerebral stroke was reported to increase by more than double in the presence of ischemic heart disease, more than triple in the presence of hypertension, more than quadruple in the presence of heart failure, and nearly quintupled when AF was present [22]. In the present study, stroke patients had statistically significant lower levels of vitamin D when compared with control group. This was concomitant with non-significant reduction of lower serum total Ca and phosphorus and increased alkaline phosphatase levels in stroke patients compared with controls. These results were in agreement with the results obtained by other studies [23,24,25,26].

The association of vitamin D deficiency with stroke severity and outcome after controlling for other risk factors suggests a role for vitamin D in the pathogenesis of stroke that could be invoked by several mechanisms. For example, vitamin D has a role in arterial hypertension via the suppression of the renin–angiotensin–aldosterone system (RAS). RAS is a key regulator of blood pressure, electrolyte, and volume homeostasis. Overactivation of the RAS system leads to hypertension which is one of the most important modifiable risk factor for all types of stroke [27, 28]. Type 2 diabetes was associated with low vitamin D levels, which was linked to pancreatic β cell dysfunction and disturbed insulin secretion [29]. In addition, vitamin D acts as a modulator of depolarization potential and stimulated insulin secretion via releasing of intracellular calcium stores [30]. Moreover, vitamin D regulates parathyroid hormone (PTH) concentration which is associated with insulin synthesis and secretion in the pancreas [29]. Vitamin D deficiency induces secondary hyperparathyroidism and increased PTH level that was associated with diabetes by causing β cell dysfunction and insulin resistance [30]. Furthermore, vitamin D deficiency was shown to be associated with dyslipidemia among stroke patients [31, 32]. It was observed that low vitamin D concentrations were associated with higher triglycerides and total cholesterol and lower levels of HDL cholesterol [31]. In this study, the results of multivariable logistic regression analysis showed that after controlling other risk factors, age, dyslipidemia, vitamin D deficiency, and large infarction size were statistically significant independent factors associated with stroke severity. This was in agreement with other studies which showed that vitamin D deficiency was associated with stroke severity [25, 33]

Regarding stroke outcome, multivariable logistic regression analysis showed that high NIHSS score, vitamin D deficiency, and large infarction size were significantly associated with mRS. Our result was in accordance with the result of other studies demonstrated that vitamin D level is a good biomarker for prognosis, functional outcome, and death in patients with acute ischemic and hemorrhagic stroke [25, 26, 33, 34]. This could be explained by the neuroprotection role of vitamin D via activation of detoxification pathways, upregulation of antioxidation/anti-inflammatory mechanisms, inhibition of inducible nitric oxide synthase, and regulation of neuronal calcium metabolism [35, 36]. Interestingly, the result of a recent experimental trial demonstrated that administration of vitamin D can attenuate infarct development after stroke probably by modulating the inflammatory response to cerebral ischemia [37].

In the current study, partial correlation of vitamin D serum level with NIHSS score severity and mRS score outcome after controlling for other variables showed a significant negative correlation between serum vitamin D level with stroke severity and outcome. Furthermore, ROC curve analysis of vitamin D serum level that best predict stroke severity was ≤ 9 ng/ml and vitamin D serum level of ≤ 17 ng/ml was the best cutoff for stroke outcome. Our finding was in agreement with other studies reported 10–12 ng/mL as the best cutoff value of vitamin D for mortality [38, 39]. However, another study estimated a higher cutoff value of serum 25(OH) D levels as indicator for early neurological deterioration to be 42.5 nmol/l [40]. These cutoff values could be used to identify patients at a higher risk of severe stroke and bad outcome that need more intensive monitoring.

Some limitations of our study include enrolling patients over different seasons. It is known that vitamin D levels exhibits some seasonal variation. In addition, we did not collect information regarding previous dietary intake and sunlight exposure. Unavailable data on serum parathyroid hormone represent another limitation.

In conclusion, our results demonstrated that vitamin D deficiency was an independent risk factor significantly associated with cerebral stroke severity and outcome after controlling other known risk factors. This result suggests a therapeutic role for vitamin D supplementation in the management of cerebral stroke. Further prospective studies are needed to verify whether correcting vitamin D deficiency may affect the severity and outcome of the stroke as well as to establish the appropriate therapeutic dose of vitamin D for supplementation in stroke patients. Screening for serum vitamin D concentrations is likely to identify individuals who are at the highest risks, particularly those with old age, diabetes, and large infarction.