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Predictive value of P300 event-related potential component in early cognitive impairment in patients with uncomplicated newly diagnosed hepatitis C virus
The Egyptian Journal of Neurology, Psychiatry and Neurosurgery volume 58, Article number: 12 (2022)
Cognitive impairment in patients with hepatitis C virus (HCV) is reported in the early onset of HCV infection without hepatic cirrhosis or marked liver impairment. Methods currently available to identify the risk for early cognitive impairment in hepatitis C virus (HCV) infection do not combine enough sensitivity and specificity. The present study aimed to evaluate the P 300 components of event-related potential (ERP) abnormalities as valid biomarkers for prediction and diagnosis of the cognitive impairment in newly diagnosed hepatitis C virus infection. This study is a case–control involved fifty patients newly diagnosed HCV and fifty age and sex-matched healthy controls. Assessments of cognitive functions were carried out by the Mini-mental State Examination, Wechsler Memory Scale Revised short form, and The Wechsler Adult Intelligence Scale, in addition to estimation of the amplitude and the latency of the P300 by the event-related potentials.
Neuropsychological scales suggested the early incidence of cognitive impairment among hepatitis C virus patients. The electrophysiological study showed significant prolongation of P300 latency and decreased amplitude in HCV patients group compared with the control group. A binary logistic regression detected that P 300 latency ≥ 369 ms was significantly accompanied by a threefold increased risk of impaired cognition (OR 3.09, 95% CI 1.59–5.72, P < 0.01), while P 300 amplitude ≤ 8.2 μv was significantly accompanied by a twofold increased risk of impaired cognition (OR 2.18, 95% 1.43–4.05, P < 0.01).
This study concluded that the P300 event-related potentials components are valid biomarker as easy, noninvasive assessment and cost-effective method of early cognitive impairment in patients with uncomplicated newly diagnosed hepatitis C virus.
Registration of Clinical Trial Research
ClinicalTrials.gov ID: NCT04389268. https://clinicaltrials.gov/ct2/show/NCT04389268
Hepatitis C virus infection is estimated to affect 3.5 million people in the USA and 71 million people worldwide [1, 2]. Hepatitis C virus is considered as epidemic infection in Egypt, having the highest prevalence in the world (14.7%) [3, 4]. In these patients, health outcomes are greatly affected by a range of common comorbidities, including various types of psychological and cognitive disorders [5, 6].
The incidence of cognitive impairment in patients with hepatitis C virus (HCV) is reported in the early onset of HCV infection without hepatic cirrhosis or marked liver impairment [7, 8]. It was found that 40–74% of cases infected with the hepatitis C virus will have one or more extra-hepatic manifestations during the disease [9, 10]. Mild cognitive impairment is among the common extrahepatic manifestations associated with hepatitis C virus without clear pathophysiological mechanism that explains its incidence .
HCV-associated neurocognitive impairment shows a prominent frontal lobe affection with impaired executive functions, disordered working memory, decision-making and impaired verbal fluency [12,13,14], disinhibited behavior and apathy , in addition to mood and emotional disturbances . The neuroimaging studies showed a remarkable affection of the frontal cortico-striatal tracts that coordinate emotion & motivation, executive functions, and motor activity . Several studies have included patients accompanied by comorbidities that may cause impaired cognition, such as liver cirrhosis, which may cause a bias that affects the results . As Kramer et al. 2002 demonstrated that patients with HCV infection showed slight but significant neurocognitive impairment, possibly indicating a further extrahepatic manifestation of chronic hepatitis C. However, our study investigated cognitive functions in newly diagnosed hepatitis C virus infection .
Event-related potentials (ERPs) are valuable clinical and research tools to investigate the cognitive functions in different neuropsychiatric diseases . The P300 is the most frequently used and defined ERPs component which is a sensitive estimate of neural activity related to attention and memory . Regardless the advanced functional neuroimaging, the ERP estimation is still considered an important tool for brain study and to investigate many neuropsychiatric disorders .
Methods currently available to identify the risk for early cognitive impairment usually investigated patients with chronic hepatitis C virus infection [22,23,24,25]. The purpose of this study is to evaluate the P 300 component of ERP alterations as a valid biomarker for diagnosis and prediction of the cognitive functions in newly diagnosed hepatitis C virus infection.
Study design and participants
This case–control study was included fifty (28 men and 22 women) first diagnosed, therapy naïve, HCV patients, aged 20–50, and recruited from the outpatient clinic and department of gastroenterology and hepatology, or referred to the department of neuropsychiatry Kafr Elsheikh University Hospital. In addition, 50 healthy controls matched for age, sex (26 men and 24 women), educational level, and socioeconomic status were included. The study was done for a 1 year from February 2019 to February 2020. A written informed consent was taken from patients and control before participation. This study was approved by the local ethical committee.
Inclusion and exclusion criteria
The inclusion criteria included newly diagnosed HCV (no current or previous treatment with interferon), uncomplicated (Child A, score 6) . The exclusion criteria were patients with cirrhosis or complicated HCV (Child B/C), causes of chronic liver disease (hepatitis B infection, alcohol abuse, parasitic infection, autoimmune disorders, cardiac failure, toxic, and hereditary) psychiatric illness, current substance abuse, renal failure, COPD, uncontrolled DM, other chronic illness that affect cognition (parkinsonism, Alzheimer's, and vascular dementia).
Clinical assessment and investigations
All participants were subjected to the following: full history taking, general medical examination, and complete neurological examination. Laboratory investigations included: HBV surface antigens, anti-HCV antibodies (HCV antibodies done by Beckman colter access immunoassay), PCR viral titer, TSH, random blood glucose, liver function, renal function, and CBC. Abdominal ultrasonography (Philips EPIC 7, Curved probe, 3.5 MHz) was done for evaluation of the liver and exclusion of hepatic cirrhosis or marked fibrosis. MRI of the brain using multiple sequences and planes without contrast on 3 T MRI closed machine to exclude neurological causes of cognitive impairment.
All patients and controls were subjected to cognitive assessment using the following tests:
Mini-mental state examination (MMSE)
This is a rapid screening estimate of cognitive functions that evaluates orientation, attention, working memory, learning, naming, construction, comprehension, and repetition. The total score is 30, with scores less than 24 indicating impaired cognition .
Wechsler Memory Scale-Revised (WMS-R) short form
The aim of the test is to provide estimates of various aspects of memory and learning functions (Rabin et al. 2005). WMS-R includes a group of subtests that evaluates figural memory, logical memory, visual paired associate, visual reproduction, and visual memory span .
The Wechsler Adult Intelligence Scale (WAIS)-IV Assessment, 2nd Edition
P300 brain wave
Auditory P300-evoked brain potential represents the brain reaction to variable sensory, cognitive, or mechanical stimuli by focusing on the diverse stimuli among the stereotypical ones (the auditory oddball paradigm) . In the auditory oddball task, persons were subjected to a series of high (1000 Hz) “task relevant” target tones and lower (500 Hz) “task irrelevant” tones of 50 ms duration, increase and decrease time, 5 ms, 85 dB, and inter-stimulus interval between 2 and 14 s. The amplitude and the latency of the P300 were estimated by the event-related potentials and the results of P 300 in HCV patients group were compared with the control group (Fig. 1). The test was conducted utilizing quantitative EMG/EP workstations using a Dantec Keypoint (Medtronic, Denmark) device. The test was performed by adding an odd-ball model, as in a set of standard "repeated" stimuli, the subject has to detect an occasionally target stimulus.
Before commencing the examination, the test was first clarified to the patient. Before the wave was registered, the test was replicated many times to ensure the patient understood the process. All of the patients were able to understand the procedure, and each examination would take about 30 min. From scalp electrodes the evoked potential waves were registered with reference electrode position on the ear lobule and a land electrode located on the hand. The active electrode is located along the midline at Oz. The reference electrode is located at location Fz. The subscript z indicates a midline position. The locations of the lateral active electrodes, O 1 O 2 PO 7, and PO 8 are indicated along with the midline active electrode location, O Z. For delivering the auditory stimulus, headphones had been used completely covered the ears. The waves have been classified as N1, P2, N2, P3, and N3 . The measured variables were the P300 latency associated with cognitive processing speed and wave amplitude associated with memory consolidation .
Data were analyzed using the Statistical Package of Social Science (SPSS) program for Windows (simple edition of the software 21). Qualitative information was described utilizing numbers and percent. Categorical variables were compared by the Chi-square test and Fischer exact test was utilized when expected cell count less than 5. Continuous variables were shown as mean ± SD (standard deviation) for parametric information and median (min–max) for non-parametric information. HCV patients group were compared with the control group by Student t test used for parametric information and Mann Whitney test used for non-parametric information.
Pearson correlation was used to correlate parametric data continuously while using Spearman correlation to correlate non-parametric data continuously. Cutoff points for P 300 latency and amplitude were determined by the ROC curve. Finally, a binary logistic regression analysis was done to establish the ability of the determined cutoff values of P300 latency and amplitude to predict the early cognitive impairment in HCV patients. The threshold of significance is set as a P value < 0.05.
No significant differences were found between the patients with HCV infection and controls regarding age, gender, marital status, occupation, level of education and socioeconomic status (Table 1).
Early cognitive impairment in HCV patients (Table 2)
Impaired cognition was detected in 7 (14%) of the patients. All subtests of the Wechsler memory test scale showed significant lower scores in cases compared with controls suggesting cognitive impairment among hepatitis C virus patients; figural memory (7.09 ± 1.19 versus 9.02 ± 0.87, P < 0.001), logical memory (26.10 ± 2.10 versus 28.48 ± 1.51, P < 0.001), visual paired association (15.76 ± 1.45 versus 17.32 ± 0.96, P = 0.025), visual Reproduction (35.02 ± 1.88 versus 39.46 ± 1.59, P < 0.001), and Visual memory span (22.04 ± 1.72 versus 25.08 ± 1.26, P < 0.001). The verbal, performance, and full scale IQ items of the Wechsler Adult Intelligence Scale were significantly impaired among HCV patients, as compared to control group (103.52 ± 6.78 versus 108.74 ± 2.67, P < 0.001 and 103.08 ± 6.16 versus 108.30 ± 3.06, P < 0.001 and 102.78 ± 5.31 versus 108.74 ± 2.55, P < 0.001, respectively). In addition, the mini-mental state examination was significantly decreased among HCV patients in comparison with the control group (23.68 ± 1.66 versus 28.40 ± 1.28, P < 0.001).
P 300 latency and amplitude in HCV patients
The electrophysiological study showed significantly prolonged P300 latency in HCV patients group compared with the control group (379.32 ± 22.73 versus 318.82 ± 12.25, P < 0.001) and reduced amplitude in HCV patients group compared with the control group (8.23 ± 1.20 versus 12.13 ± 0.93, P < 0.001) (Fig. 2).
Correlation between P300 and cognition
The increase of P 300 latency was associated with decrease of all the subtests of Wechsler memory test scale, Wechsler Adult Intelligence Scale, and MMSE. There was a significant negative correlation between P300 latency and all subtests of Wechsler memory test scale; figural memory (r = − 0.48, P = 0.02), logical memory (r = − 0.41, P = 0.03), visual paired association (r = − 0.39, P = 0.04), visual reproduction (r = − 0.67, P = 0.001), and visual memory span (r = − 0.63, P = 0.002). In addition, there was a significant negative correlation between P300 latency and all subtests of Wechsler Adult Intelligence Scale; verbal IQ (r = − 0.52, P = 0.01), performance IQ (r = − 0.54, P = 0.01), full scale IQ (r = − 0.68, P = 0.001). Similarly, there was a significant inverse correlation between P300 latency and MMSE (r = − 0.66, P = 0.001) (Table 3).
The decrease of the P300 amplitude was associated with decrease of all the subtests of Wechsler memory test scale, Wechsler Adult Intelligence Scale, and MMSE. There was a significant positive correlation between P300 amplitude and all subtests of Wechsler memory test scale; figural memory (r = 0.45, P < 0.05), logical memory (r = 0.42, P < 0.05), visual paired association (r = 0.37, P < 0.05), visual reproduction (r = 0.61, P < 0.005), and visual memory span (r = 0.54, P < 0.01). In addition, there was a significant positive correlation between P300 amplitude and all subtests of Wechsler Adult Intelligence Scale; verbal IQ (r = 0.51, P < 0.01), performance IQ (r = 0.50, P < 0.01), full scale IQ (r = 0.63, P < 0.005). Similarly, there was a significant negative correlation between P300 amplitude and MMSE (r = − 0.60, P < 0.01) (Table 4).
Prediction of the impairment of cognition
The cutoff value of the P 300 latency was equal to 369 ms with sensitivity and specificity more than 70% and the cutoff value of the P 300 amplitude was equal to 8.2 μv with sensitivity and specificity more than 70% (Table 5). The total performance of the cutoff values detected by the ROC curve for the P 300 latency was 0.89 (95% CI 0.72–0.95) and the P 300 amplitude was 0.84 (95% CI 0.68–0.89) (Fig. 3). Finally, binary logistic regression demonstrated that P 300 latency ≥ 369 ms was significantly accompanied by a three times an increase of impaired cognition (OR 3.09, 95% CI 1.59–5.72, P < 0.01), while P 300 amplitude ≤ 8.2 μv was significantly accompanied by a two times an increase of impaired cognition (OR 2.18, 95% 1.43–4.05, P < 0.01) (Table 6).
Hepatitis C virus (HCV) infection is a major health problem around the world . The neurocognitive impairments in HCV are frequently subclinical , mostly happen in patients infected with HCV independent of the severity of the hepatic condition or the activity of HCV infection , and precedes the process of cirrhosis and were not related to the viral load titer or the genotype [35, 36]. Impaired cognition in HCV infection has been found in many studies [7, 8], but few reports not confirming such an association. Cordoba et al. 2003 and Soogoor et al. 2006 reports denied any correlation between HCV and cognition [37, 38]. However, Córdoba et al. collected his sample from blood donation patients, while Soogoor et al. had a very young aged sample which means the two cases are in the early phase of the illness. In addition, Abrantes et al. 2013 failed to demonstrate proof of a relationship between HCV infection and impaired cognition. However, the few number of patients included in this study may have influenced the results .
Our study investigated the cognitive impairment in newly diagnosed HCV patients with no comorbidities or liver cirrhosis that can affect cognition. To our knowledge it is the first study to determine the cut off values of P300 latency and amplitude for prediction of early cognitive impairment in newly diagnosed HCV patients.
Early cognitive impairment in HCV patients
In our study, mild impaired cognition was detected in 7 (14%) of the patients and the mini-mental state examination was significantly lower in HCV patients compared to normal control (23.68 ± 1.66 versus 28.40 ± 1.28, P < 0.005). Similarly, a study of a large population of chronic HCV patients has revealed the incidence of subclinical impaired cognition in 18% of patients . While other studies found a higher cognitive impairment in about one-third of HCV individuals [41,42,43]. Our study included only the newly diagnosed HCV patients and a younger age group (20–50), with the exclusion of liver fibrosis & cirrhosis, and drug abuse, so it was expected that cognitive impairment will be less than the previous studies that included more chronic hepatitis, cirrhosis, and older age patients, for example, Monaco et al. 2015 included in their study patients with advanced liver impairment, alcohol intake, and drug abuse .
In our study, the Wechsler Adult Intelligence Scale was significantly impaired among HCV patients and all subtests of the Wechsler Memory scale were significantly diminished in HCV compared to normal controls (P < 0.001). Similarly the study of Weissenborn and his colleagues 2004 found that the mean WAIS-R verbal score was significantly higher in the healthy controls than in the HCV infected patients . In addition to the study of Abo Hagar and his colleagues 2018 detected that all subtests of the Wechsler Memory Scale were statistically higher except verbal memory score .
Event-related potentials (ERPs) are safe and simple tools for examining the processing of data and cognitive functions, such as attention, learning, memory, and decision-making [44, 45]. Sutton et al. 1965 were the first to reveal evoked potentials with its peak amplitude at about 300 ms and detected a strong relationship between evoked potentials and cognition . In this manner, it has been distinguished by numerous investigations that impaired cognition in different brain diseases, such as Alzheimer dementia, Parkinsonism, epilepsy, and cerebrovascular stroke, can be evaluated by ERPs [47,48,49,50].
P 300 latency and amplitude in HCV patients
In our study, the electrophysiological study showed significantly prolonged P300 latency in HCV patients group compared with the control group (379.32 ± 22.73 versus 318.82 ± 12.25, P < 0.001) and reduced amplitude in HCV patients group compared with the control group (8 ± 1.20 versus 12.13 ± 0.93, P < 0.001). Likewise, Kramer et al. 2002 found that 16% of patients of HCV had abnormal P300 latencies not related to the hepatic severity. This finding may reflect impaired information processing speed, reaction time, and the attention paid to a stimulus .
P 300 and cognition in HCV patients
ERPs are sensitive procedure more than psychometric tests in recognition of initial changes in the brain function of hepatic patients, that is why this neurophysiological approach has to be applied clinically . In our study, the increase of P 300 latency was associated with a significant negative correlation with all the subtests of the Wechsler memory test scale, Wechsler Adult Intelligence Scale, and MMSE. While there was a significant positive correlation between the decreased P300 amplitude and all the subtests of the Wechsler memory test scale, Wechsler Adult Intelligence Scale, and MMSE. The amplitude and latency of the P300 wave give data about the processing of the cognitive functions . Longer latencies mean inferior mental performance relative to shorter latencies . P 300 amplitude appears to reflect stimulus data processing and the more attention produces larger P 300 waves [53, 54]. ERP establishes a millisecond-by-millisecond recording of neural processing of certain responses, such as sensory encoding and working memory. Accordingly, it gives a noninvasive tool to assess the brain functions in cognitive disorders . The utilization of P300 for estimation of brain information processing avoids the bias of certain factors, for example, fatigue or depression that can affect the neuropsychological tests. In addition, P300 considers a sensitive marker of impaired higher cognitive functions [36, 51].
In our study, the cutoff value of the P 300 latency was equal to 369 ms and the cutoff value of the P 300 amplitude was equal to 8.2 μv with sensitivity and specificity more than 70%. P 300 latency ≥ 369 ms was accompanied by a three times an increase of impaired cognition and P 300 amplitude ≤ 8.2 μv was accompanied by a two times an increase of impaired cognition.
Limitations of the study
The small size of the sample, the absence of liver biopsy as an important tool of evaluation of liver state, and the absence of advanced functional neuroimaging were a group of study limitations that should be considered in the future work about that issue.
Our findings suggested that the combined use of sensitive neuropsychological scales and the analysis of the P300 may offer a valuable method for the diagnosis of impaired cognition in patients with HCV. P300 event-related potentials components are valid biomarker as easy, noninvasive assessment and cost-effective method of early cognitive impairment in patients with uncomplicated newly diagnosed hepatitis C virus.
Availability of data and materials
The datasets generated and analyzed during the current study are not publicly available due to institutional limitations, yet they are available from the corresponding author on reasonable request.
Complete blood count
Hepatitis B virus
Hepatitis C virus
Mini-mental state examination
Magnetic resonance imaging
Polymerase chain reaction
Statistical Package of Social Science
Wechsler Adult Intelligence Scale
Wechsler Memory Scale-Revised
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All procedures performed in this study were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration. Informed written consent was obtained from all individual participants included in the study. This study was approved by Mansoura Faculty of Medicine Institutional Research Board ("MFM-IRB").
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Esmael, A., Belal, T., Amer, I.F. et al. Predictive value of P300 event-related potential component in early cognitive impairment in patients with uncomplicated newly diagnosed hepatitis C virus. Egypt J Neurol Psychiatry Neurosurg 58, 12 (2022). https://doi.org/10.1186/s41983-022-00450-4
- Hepatitis C virus
- Cognitive impairment
- Event-related potentials
- P300 latency
- P300 amplitude