Fig. 2

CBL treatment improves histo-architecture including viable motor neurons and astrocytes count at the site of spinal cord injury. a1–a3 Negative control group, b1–b3 CI group, c1–c3 CI + 7 days CBL group and d1–d3 CI + 21 days CBL group showing: Viable multipolar neurons (black thick arrow), oligodendrocytes nucleus (discontinuous black arrow), astrocytes nuclei (black arrowhead), homogenous neuropil (NP), blood vessel (black vertical arrow), intact axon fiber (green arrowhead), intact axon tract (green arrow),viable neuron with mottled Nissl granules (blue thick arrow), dark debris of dead neurons (blue arrow), cavities enclosed remnant of degenerated neurons (vertical blue arrow), edematous blood vessels (blue arrowhead), dishomogenous and edematous neuropil (inside black circle), degenerated axons fibers (green arrow with double head),disrupted axon tracts (corrugated green arrow), neurons with chromatolysis (black tailed arrow) or with clumped Nissl granules (green thick arrow). A Quantification of viable motor neurons count showing improvement following 21-day CBL treatment at the site of injury and B Quantification of astrocytes count showing CBL treatment—both 7 and 21 days—showed reduced astrogliosis at the site of injury. Data are expressed as median and interquartile range following Kruskal–Wallis test followed with Dunn’s multiple comparison test for A, and mean ± SEM following one-way ANOVA test followed with post hoc Tukey test for B